Flexibility to read a variety of plate formats. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories. Step 2: Launch SoftMax Pro Software. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. #0200-6182). Our systems for high-content imaging (HCI) and high-content analysis (HCA) provide flexible scalability making it easy to evolve your system alongside your research. D. Skip to main content 美谷分子仪器（上海）有限公司 咨询服务热线：400-821-3787. We would like to show you a description here but the site won’t allow us. Viewing results on your new SpectraMax iD3 Multi-Mode Microplate Reader. 1). What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for. Plate section without PathCheck applied. #6590-0131) Fluorescence Filter with holder 625nm BW 35nm (Molecular Devices cat. 561. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. ABS Test results. View SoftMax Pro GxP. Products : General, FilterMax F5 reader. DOCUMENTATION. com Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e. Increased throughput. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for. San Jose, California, June 5, 2018. The 1/absorptivity value for double stranded DNA at 260 nm is commonly assumed. The Bauer Core Facility at Harvard University offers of variety of plate-reader choices to its users (mostly from Molecular Devices), and the SpectraMax® L is. Uncontrolled Copy for Training and Reference Purposes Only . Page 16 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide To exchange an excitation or emission filter in a slide: 1. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. The small footprint and touchscreen control allows for easy laboratory setup with minimal investment of time, cost, and effort. Due to their ability to simultaneously measure multiple wells in a single run, fluorescence plate readers are useful for conducting various assays, such as quantitative assays, enzyme assays, and ELISAs. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. 6 % of reading 4. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. Molecular Devices' microplate readers. 150 μL of MTT solvent was then added to each well to solubilize the formazan product, and the plate was placed on a shaker for 15 minutes. Supersedes 1. To update the driver. For biochemical assays, the washer removes unbound material and unreacted reagents. a single plate read using any standard cuvette, or 96- or 384-well microplate. Step 3. #I3X), equipped with the following: HTRF Detection Cartridge (Molecular Devices cat. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Molecular Devices provides web-based tutorials (webinars) and training courses throughout the year. 384 deep well plate: packed in sleeves of 6 , 24 per case. The Gemini instruments can read some luminescence but it is about 100 times less sensitive than a dedicated luminometer. HepG2 cells were plated into a 96-well clear-bottom, white-walled plate at 10,000 cells/well and incubated at 37°C. 1. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Open the top cover of the instrument. It may be named "Pothos". Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Plate pre-read measurements were made in a Costar UV plate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). 과 같은 Parameter를 검사하여 평가할 수 있습니다. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Chapter 1: Introduction The SpectraMax iD3 Multi-Mode Microplate Reader from Molecular Devices is a monochromator-based, multi-mode plate reader. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. Vmax® plate reader specifications. With SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 1 up to 3600 sec. Eight filter options provide simplified assay setup. Better absorbance accuracy. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. integration time per well, depending on firmware version. Step 2. As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. SOP Title: Use and Maintenance of a Molecular Devices M5 Plate Reader Document ID: HSL_EQ_005 Version 2. 0 . 7°C (gradient heating design). #iD3) Methods. We would like to show you a description here but the site won’t allow us. . Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and. We therefore gathered salient. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 9. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. These systems are hybrids that allow you to choose between. Endpoint. Avoid losing high signal. together with a Molecular Devices plate. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial. Search. Molecular Devices SpectraMax 384 Plus Microplate Reader. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Purchase a new SpectraMax Multi-Mode Microplate Reader and. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. To update the driver. SpectraDrop High-Throughput Screening (HTS) Kit. #i3x) SpectraMax L Microplate Reader (Molecular Devices cat. Details. Also refer to the Multimode Reader Comparison Chart Features. Firmly grasp the snap connector beside the snap, then push the snap down. Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. PBS was removed, and cell pellets were. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. Narrow bandwidths deliver increased measurement accuracy and linearity over the widest range of assays, including. SpectraMax® Paradigm Plate Reader Oksana Sirenko, Cathy Olsen, Vanessa Ott and Evan F Cromwell Molecular Devices, LLC, 1311 Orleans Drive, Sunnyvale, CA 94089 There has been a trend in the past decade from biochemical assays toward cell based assays. On the back, in the right hand top corner, there. The drawing shows a standard microplate of 15 mm height covered with a lid. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. ; Page 2 Molecular Devices equipment, software, reagents, and consumables. Note: removal of medium, followed by addition of a 1X solution of calcein AM and EthD-III, is. on the similarities in performance between four single-mode absorbance plate readers: SpectraMax® ABS, SpectraMax® ABS Plus, SpectraMax® 190* and VersaMax™* Microplate Readers. Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. or 0. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. The adapter is required for optimum performance with standard 96-well and 384-. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. The VMax® Microplate Reader is capable of reading up to two wavelengths at a time in the visible wavelength spectrum (400nm–750nm), with 4 filter choices included at time of purchase. Published Dec 16, 2020 | Updated Dec 16, 2020. These lab workhorses are used in drug discovery. M5. Our microplate readers and software are the industry’s most cited and have empowered life science researchers to advance protein and cell biology. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. 2. book Page 1 Wednesday, October 12, 2011 4:56 PMThe SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. begin reading microplates with seven mouse clicks. Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. SAN JOSE, Calif. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. Fluorescence Microplate Reader Comparison Chart. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. Installation Qualification (IQ)the iD5 reader’s filter system. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax®. in steps of 0. It features a modular design that allows users to add or change detection cartridges according to their needs. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 76358-624. W×D×H. Clone Screening. Gemini XPS and EM Microplate Readers with dual monochromator fluorometers provide a flexible environment to determine the optimal excitation and emission settings for fluorescence intensity assays. The instrument settings were identical to those used in Example 1 above. Combining multiple microplate technologies and detection modes into a single, more versatile unit may be an ideal choice especially if you have. Five-mode microplate reader with automatic filter identification and western blot capability The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence. B. Multi-mode Microplate Readers. When the user applies a set temperature of 37°C, the top is heated to 37. SpectraTest Multi-Mode Validation Plate User Guide SpectraTest Validation Plates and SoftMax Pro Software reader validation protocols. The SpectraMax® 340PC384 Reader from Molecular Devices provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro Data Acquisition and Analysis Software. com #THX1138 Introduction The proteasome is a massive protein complex inside all eukaryotic cells (and some bacteria) that removes. Luminescence reading is a secondary mode of the Gemini instruments. +1 800-635-5577. The SpectraMax ABS Plus reader utilizes Molecular . Keywords: spectramax ABS microplate readers, spectramax ABS plus microplate readers, spectramax ABS readers, ABS plus microplate readers, ABS. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Double-click the unknown device with the yellow warning icon. The Beacon Melamine Plate Kit is a competitive enzyme-linked immunosorbent assay (ELISA) for the quantitative analysis of melamine in contaminated samples. SoftMax Pro GxP Software. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. Uncontrolled Copy for Training and Reference Purposes Only . On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. ORDER. Cell line development teams will realize savings on time and resources,SpectraMax i3x. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Superior optical system includes a xenon flash lamp and features. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. TECHNICAL NOTE. 0 OD for earlier models. SpectraMax ABS and SpectraMax ABS Plus Microplate Spectrophotometers User Guide Temperature Regulation When you power on the instrument the incubator is off. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. Multimode microplate readers offer flexibility, and ease of use. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. Introduction. Select the Driver tab. Ultraviolet (UV) measurements in microplates became possible when Molecular Devices introduced the first UV-capable microplate reader. Use a Phillips screwdriver to remove the screws, and then remove the plate from the instrument. and California) Microplate Spectrophotometer 100-240V ~ 4A 50-60 HZ T4. Our SpectraMax microplate readers and SoftMax Pro software are the industry's most cited and have empowered life science researchers to advance protein and cell biology―breaking the. 9. GxP. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. FAST AND ACCURATE METERTECH Microplate Reader $3,650. 40266 Email: drugdiscoverytech@lifetech. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. SpectraMax Microplate Readers. Remove the lamp screw (on the right side of the lamp, as shown in the diagram) then gently pull the connecting wires to remove the lamp from the body of the instrument. StakMax Handling Stacker Base Unit. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. #S1207. 7°C (gradient heating design). 1 sec. Supplier: Molecular Devices. Access all the resources, tools and information you need to stay productive. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. Molecular Devices provides the complete solution covering the instrument and software: • SpectraTest® ABS1, FL1, and LM1 Validation Plates for hardware validation of absorbance, fluorescence, and luminescence modes • IQ/OQ for all microplate readersSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Single-mode Microplate Reader. A. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The wavelength required to excite the sample can be selected using either a filter of a specific wavelength or a monochromator tuned to the required. One plate carrier adapter is provided with the instrument. The drawing shows a standard microplate of 15 mm height covered with a lid. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 996 (n = 3) ; for SpectraMax Plus 384 reader, r 2 = 0. Tests every subsystem of the reader or . Plate stacker and robot integartion. visible absorbance, fluorescence. 0200-6263. UV/Vis spectrophotometer & microplate reader with built-in cuvette port. However, when a specific probe is not available, or it causes off-target effects on the. Fill a clean plate with water. These plate readers in the list below, are no longer included with the new SoftMax Pro 6 reader menu. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. ®This document is provided to customers who have purchased Molecular Devices, Inc. Single- and multimode microplate readers are useful for very broad range of applications including ELISA, kinetics and high throughput screening assays. Instruments are configurable for the assay-specific detection modes and performance your laboratory needs. Multimode Microplate Readers. The adapter is required for optimum performa nce with standard 96-well and 384-. SoftMax® Pro 7. Figure 1. Select Start>Control panel>Device Manager. Flexibility to read a variety of plate formats. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular. Cellular Imaging Systems. Get the most out of SoftMax Pro. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented. By measuring optical pathlength, it eliminates the last. 더 많은. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Monday to Friday 7am - 5pm Pacific Time. SpectraMax® M5/M5e Multimode Plate Reader, Molecular Devices Supplier: Molecular Devices Description: The SpectraMax® M5 Multi-Mode Microplate Reader delivers. Page 1 of 8 . A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. A plate reader’s software may offer automated analysis using protocols configured for a particular assay. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. 1. #4TI-0655) Microplates. EMax. The resulting OD 426 values are visually represented above as a color map generated in the plate section of SoftMax. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. The SpectraTest® ABS1 Absorbance Validation Plate tests optical performance using NIST-traceable standards. Plate reader. Microplate Readers. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. 5 minutes (ValitaTiter) 15 minutes (ValitaTiter PLUS) Integrating ValitaTiter and Molecular Devices fluorescence polarization (FP) plate readers produces a seamless IgG titer assay setup and optimization. SpectraMax Microplate Readers. Luminescence was read every five minutes for five hours with three seconds of orbital shaking before each read. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated— any potential issues are. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. The BCA assay plate was read at 562 nm, the original wavelength setting in the protocol, while for the BCA Rapid. 5 sec. book Page 1 Tuesday, July 6, 2010 2:08 PM SpectraMax Microplate Readers. Our luminescence plate readers, also referred to as luminometers are ideal for measuring a wide range of assays including dual luciferase reporter gene, G protein-coupled receptor (GPCR) via aequorin, bioluminescence resonance energy transfer. SoftMax Pro’s 21 curve fit options, including 4-parameter logistic and 5-parameter logistic models, and the ability to assign multiple standard curves within the same plate make it easy to interpolate unknown samples or perform parallel line analysis (PLA) to determine relative potency. For the name of the nearest authorized Molecular Devices Corporation service facility, please contact Molecular Devices at one of the following telephone numbers: (408) 747-1700 (800) 635-5577 (U. A standard spectrophotometer measures the absorbance of one sample at a time. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. ” So, acquisition and analysis come from one software system. 1. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. #P7589 or P11496) • Black 96-well plate (Greiner Bio-One, cat. to that of dedicated single-mode readers. Microplate readers from Molecular Devices are designed to provide consistent performance for many years. First, as mentioned before, the walkaway time significantly. Timothy Bolus, MPM, MBA, Compliance Program Manager. For cryo preservation cells were scaled up in 10-layer factories (Corning Cat. 7°C and the bottom to 36. The PathCheck Sensor is a revolutionary new awardwinning1 technology in SpectraMax® Instruments for measuring the fluid level in every well of a microplate 2–5. 0 . A kinetic plate read was initiated at time zero. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. Imaging System Giles Scientific Biomic V3 Automatic Plate. The computer controlling the plate reader runs Windows XP. 2 only) SpectraMax M-series Microplate readers. #0200-7009) Optimal instrument settings for ValitaTiter (P/N VAL003 or VAL013, left table) and ValitaTiter Plus (P/N VAL004 or VAL014, right table) assays on the SpectraMax i3x reader are shown below. SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models. Plate stacker and robot integration The Gemini EM Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. With optimized reagents, option to operate the. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode Detection Platform. for SoftMax Pro Software. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. the iD5 reader’s filter system. The SpectraMax 190 reader can be integrated with Molecular . Every step is optimized to simplify analysis and reporting to support our microplate readers. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. The Back Panel Figure 1. performs within its specifications. Patented pathlength correction for better absorbance accuracy Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical pathlength) of samples with no temperature dependency using the patented PathCheck® Sensor technology. Step 1: Capture antibody binds to ELISA plate wells. Additional high-quality filters to. The CellTiter-Glo assay from Promega uses luciferase. In . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Absorbance, fluorescence, and luminescence detection with upgradeable high-performance capabilities. The instrument reads any standard cuvette, microcuvette, test tube or 96- and 384-well standard or UV transparent microplate over a full 190 to 1000. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. 314 low lamp level warning. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Cell Cytotoxicity assay procedureMolecular Devices. Multi-mode Plate Reader. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. SpectraMax® 190 Absorbance Plate Reader, Molecular Devices. View SoftMax Pro GxP. Plus 384. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Plate reader settings used for data acquisition of bacterial growth curve. Consider non binding surface type plate (Greiner and Corning are 2 manufacturers)On SpectraMax L, 1 RLU = 1 count per second for photon counting mode only. 9. After centrifugation, the plate was immediately read on the M5e and i3x readers using the instrument settings listed in Table 1. A. An IL-22 standard curve was prepared and a sandwich ELISA performed using the MultiWash+ plate washer in strip mode to wash the wells. Fluorescence Microplate Reader Comparison Chart. The SpectraTest® ABS1 Absorbance Validation Plate**4 °C above ambient to 45 °C. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Reader Documentation Version No. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The Molecular Devices SpectraMax 384 Plus is a UV/Vis spectrophotometer and microplate reader combination for more reliable and fast data reporting with built-in cuvette port and microplate drawer. SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. Some familiarity with Windows should be sufficient to use it. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Shop now. The reader measures both flat and round 96-well microplates and. Plate layout configuration. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. Page 28 Chapter 5: Read Modes and Read Types 1. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. “Sensitivity is pretty much clutch for luminescence,” says Daly. Sale price $18,499. Open the Protocol Library, locate the ‘Protein Quant’ folder, and open the pre-configured protocol ‘NanoOrange’. An end-to-end solution for high-content imaging and analysis. Sale price $3,495.